To achieve the challenging tasks of Targetfish, we brought together 30 partners from 10 EU member states, 2 associated countries and 1 International Cooperation Partner Country (Chile).
1. Wageningen Universiteit, Netherlands
Principal investigators: Geert Wiegertjes, Maria Forlenza, Carmen Embregts
Research focus: characterization of the immune responses induced by adjuvants and the development of vaccines against CyHV3 and SVCV.
2. Aarhus University, Denmark
Principal investigators: Niels Lorenzen, Ellen Lorenzen, Jesper Skou Rasmussen, Dennis Bela-ong, Dagoberto Sepulveda
Research focus: screening of novel expression systems for antigens and mass delivery of DNA vaccines to fish, responsible for performing in vivo experiments with VHSV in rainbow trout and turbot.
3. The University Court of the University of Aberdeen, UK
Principal investigators: Chris Secombes, Sam Martin, Jun Zou, Tiehui Wang
Research focus: studying the potential role of fish cytokines as vaccine adjuvants, the use of bacteriophages for vaccine and adjuvant delivery and the effects on T cell polarisation and cellular immunity in protection.
4. The Scottish Ministers Acting Through Marine Scotland, UK
Principal investigators: Bertrand Collet, Catherine Collins
Research focus: optimising a vaccine for PDV in Atlantic salmon, exploring T cell modulating cytokines as adjuvants and establishing non lethal tests for the prediction of efficacy.
5. Friedrich Loeffler Institut – Bundesforschungsinstitut Fuer Tiergesundheit, Germany
Principal investigators: Uwe Fischer, Takuya Yamaguchi
Research focus: vaccine development in rainbow trout and carp, including the efficacy testing of DNA-vaccines and baculoviral vector vaccines for mass delivery. Provide and test DAMP based adjuvants and study cell-mediated immune responses.
6. Instituto Nacional De Investigacion Y Tecnologia Agraria Y Alimentaria, Spain
Principal investigators: Carolina Tafalla, Jaime Pignatelli Garrigós
Research focus: optimization/ prediction of efficacy of VHSV vaccines in rainbow trout and turbot, also studying immune mechanisms that may correlate with protection in these species.
7. Universitat Autonoma de Barcelona, Spain
Principal investigators: Oriol Sunyer, David Parra
Research focus: dissecting the mucosal humoral and cellular immunity in the protection conferred by effective immersion and oral vaccines.
8. Universita Degli Studi Della Tuscia, Italy
Principal investigators: Giuseppe Scapigliati, Francesco Buonocore, Simona Picchietti
Research focus: experimental work related to sea bass by including novel vaccination strategies for VERV, use of adjuvants, and studies related to the immune responses in correlation with protection in this species.
9. Institut National De La Recherche Agronomique, France
Principal investigators: Pierre Boudinot, Christelle Langevin, Susana Magadán
Research focus: dissection of the immune response in successful vaccination strategies in salmonids, with a special focus on the mechanisms that condition long-term memory in fish.
10. Norges Veterinaerhogskole, Norway
Principal investigators: Øystein Evensen, Hetron M. Mungang’andu
Research focus: IPNV and PD virus immunization studies, design of adjuvants for immersion delivery (IPNV) and vector design (PD virus).
11. The University Of Stirling, UK
Principal investigators: Alexandra Adams, Kim Thompson, Rowena Hoare
Research focus: testing and monitoring vaccine efficacy, development and testing of a vaccine to prevent RTFS in rainbow trout and Atlantic salmon, including the testing of various adjuvants and novel mass delivery methods.
12. Istituto Zooprofilattico Sperimentale delle Venezie, Italy
Principal investigators: Calogero Terregino, Anna Toffan
Research focus: perform VERV/VNN vaccination /challenge trials under controlled conditions. Collecting identified tissues during the experiments and submitted to relevant partners for further investigations.
13. Københavns Universitet, Denmark
Principal investigators: Kurt Buchmann, Per W. Kania, Moonika H. Marana, Jiwan Kumar Chettri
Research focus: contribute to production of a subunit vaccine targeting furunculosis in mariculture of rainbow trout.
14. Výzkumný ústav veterinárního lékarství, Czech Republic
Principal investigators: Tomáš Veselý, Dagmar Pokorová
Research focus: VRI will be involved especially in the experimental infections to be performed in carp.
15. The Hebrew University of Jerusalem, Israel
Principal investigators: Lior David, Roni Tadmor
Research focus: Cy-HV3 vaccination of carp families from various strains using several vaccines. The vaccinated fish will be challenged with the virulent virus to test the vaccination efficiency.
16. University Murcia, Spain
Principal investigators: Victoriano Mulero, Jorge Galindo-Villegas
Research focus: development of adjuvants in WP3 and their validation and testing for seabream.
17. Tethys Aquaculture Limited, UK
Principal investigators: Patrick Smith
Research focus: amplifies the research outputs in products and services that fit the aquaculture industry needs.
18. PatoGen Analyses AS, Norway
Principal investigators: Vidar Aspehaug, Magnus Devold
Research focus: development of Real-Time PCR-assays for detection and typing of pathogens, Q-PCR assays for differentiation between pathogen variants, & Q-PCR assays for differentiation between vaccinated and infected fish.
19. Fishlab, Denmark
Principal investigators: Kirsten Engell-Sørensen, Louise Hjorth Nørremark
Research focus: supply healthy, vaccinated and non-vaccinated turbot larvae, fry as well as eggs for experimental purposes, will optimize different vaccination protocols and will develop oral vaccination methodology by use of bioencapsulation using live feed (two species of calanoid copepods, Artemia and rotifers).
20. Naxo OÜ, Estonia
Principal investigators: Juri Sober, Indrek Toots, Kaia Talts
Research focus: developing monoclonal antibodies against different fish viruses, as well as antibodies necessary to study protective immune responses in the different fish species addressed by TargetFish.
21. Ridgeway Biologicals, UK
Principal investigators: Tim Wallis, Terry Field
Research focus: carry out experiments characterising the secreted proteomes of Edwardsiella tarda. Responsible for the culture, expression and purification of antigens p and blending them with the appropriate adjuvants identified into prototype vaccines.
22. Rossi A/S, Denmark
Principal investigators: Torben Rød
Research focus: develop vaccination machines of fry. New working procedures as well as injection machines for small-size fish will be optimised, provided and implemented.
23. Ingeniatrics Tecnologias S.L., Spain
Principal investigators: María Flores
Research focus: participate in the microencapsulation of different fish vaccines for oral or immersion vaccination in alginate derivatives.
24. BigDNA, UK
Principal investigators: John March
Research focus: developing a strategy for the delivery of antigens for different fish vaccines through the use of bacteriophages.
25. W42 Industrial Biotechnology GmbH, Germany
Principal investigators: Ansgar Stratmann, Ulrich Giesecke
Research focus: support the development of production strains for different virus-proteins through the development of different purification/enrichment strategies.
26. Aquark, Greece
Principal investigators: Panos Christofilogiannis, Joanna Tavla
Research focus: field testing and design of effective vaccination strategies and delivery protocols.
27. CentroVet, Chile
Principal investigators: David Farcas, Jaime Tobar
Research focus: establishment of oral vaccination strategies for different fish pathogens using the
MicroMatrix™ Technology (ABN).
28. Danish Aquaculture Association, Denmark
Principal investigators: Niels Henrik Henriksen
Research focus: participate in the field trials foreseen in TargetFish.
29. BioMar A/S, Denmark
Principal investigators: Anne Hjørngaard Lauritsen, Ralph Bickerdike, Trygve Sigholt
Research focus: collaborate in the development of oral vaccination strategies and delivery methods for different fish vaccines.
30. Bionaturis, Spain
Principal investigators: Juan José Infante, Ana de la Heras, Rosa Osuna
Research focus: development and production of VHSV and nodavirus antigenic proteins by using its proprietary platform FLYLIFE to develop oral vaccines against these viruses.